Develop your own ELISA Assays

Features Coated Plates, Blocking and Wash Agents & Detection Reagents

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The Assay Development Guide focuses on the development of ELISA, a key research tool, with a brief overview of ELISA and key protocols.  The guide features a vast selection of pre-coated plates, blocking agents, detection substrates and labeling reagents, including fluorescent dyes

The ELISA Development Handbook features the following sections:

• Introduction
  • Featuring an overview of the ELISA principle and a general ELISA protocol
• Coated 96 well plates
  • Available in clear, black or white for colorimetric, fluorescent and chemiluminescent assays respectively.
  • Plates for Antibody Binding
    • Coated with Protein A, Protein G, Protein A/G, Protein L or α-rabbit or mouse antibodies
  • Plates for Biotin Binding
    • Designed to immobilize biotinylated biomolecules through Neutravidin and Streptavidin
  • Plates for Protein and Peptide Binding
    • Bind your protein or peptide of choice through their primary amines or sulfhydryls. Alternatively, couple GST or 6X His tagged proteins to glutathione and nickel coated plates.
• Blocking Agents
  • Large selection of blocking agents, including non-animal blocking agents, non-sera animal protein blockers and other protein blocking agents
• Washing Agents
  • PBST, TBST and a large selection of proteomic grade detergents
• Detection Probes
  • Enzyme conjugated secondary antibodies with horseradish peroxidase (HRP) and alkaline phosphatase (AP)
• Antibody Labeling
  • Fluorescent dye labels and biotin labeling reagents
• Detection Substrates
  • Colorimetric and chemiluminescent enzyme substrates for detection