2D Sample Preparation
2D Electrophoresis is Sensitive to Reagents Used in Protein Extraction
Improper sample preparation results in poor data!
Many of the chemicals and reagents required for cell lysis interfere with downstream process, including:
- 2D & 1D Electrophoresis
- Western Blotting
- Mass Spectrometry
- Enzyme Assays and other ELISAs
The most common interfering agents are reducing agents (i.e. DTT), detergents (i.e. CHAPS), chaotropes (i.e. urea) and salts (i.e Sodium Chloride).
Download application for more details.