Immobilization of Antibodies & Glycoproteins to Agarose Resin
Addition of a Catalyst Improves Coupling by >20%
Affinity chromatography is a key research tool for the purification of known ligands, including antigens and proteins, and, as an extension, identification of novel interacting partners.
Numerous methods exist for the generation of affinity columns, using various ligands and coupling chemistries. One important tool is the covalent coupling of glycoproteins to reactive hydrazides immobilized on a solid support (agarose beads). This technique is routinely used for the immobilization of monoclonal and polyclonal antibodies with glycosylated Fc domains, as the resulting immobilized antibodies have unobstructed antigen-binding domains.
This application note reviews the coupling chemistry for covalently linking sugars to reactive hydrazides and evaluates the use of a chemical catalyst to improve coupling.
Download the free application note to read more about the catalyst for improved coupling!