Straightforward Optimization of Detergent Concentrations
A Colorimetric Estimation: Affordable & Simple, But Effective
The Critical Micelle Concentration, or CMC, is very important to researcher's as it allows them to use the correct amount of detergent. Too little means inadequate solubilization of proteins and too much can affect downstream process and problematic detergent removal steps.
CMC can be determined by:
- Light scattering
- Surface tension
- Dye Solubilization