Develop your own ELISA Assays

Features Coated Plates, Blocking and Wash Agents & Detection Reagents


ELISA Assay Development Protocol and GuideThe Assay Development Guide focuses on the development of ELISA, a key research tool, with a brief overview of ELISA and key protocols.  The guide features a vast selection of pre-coated plates, blocking agents, detection substrates and labeling reagents, including fluorescent dyes

The ELISA Development Handbook features the following sections:

  • Introduction
    • Featuring an overview of the ELISA principle and a general ELISA protocol
  • Coated 96 well plates
    • Available in clear, black or white for colorimetric, fluorescent and chemiluminescent assays respectively.
    • Plates for Antibody Binding
      • Coated with Protein A, Protein G, Protein A/G, Protein L or α-rabbit or mouse antibodies
    • Plates for Biotin Binding
      • Designed to immobilize biotinylated biomolecules through Neutravidin and Streptavidin
    • Plates for Protein and Peptide Binding
      • Bind your protein or peptide of choice through their primary amines or sulfhydryls. Alternatively, couple GST or 6X His tagged proteins to glutathione and nickel coated plates.
  • Blocking Agents
    • Large selection of blocking agents, including non-animal blocking agents, non-sera animal protein blockers and other protein blocking agents
  • Washing Agents
    • PBST, TBST and a large selection of proteomic grade detergents
  • Detection Probes
    • Enzyme conjugated secondary antibodies with horseradish peroxidase (HRP) and alkaline phosphatase (AP)
  • Antibody Labeling
    • Fluorescent dye labels and biotin labeling reagents
  • Detection Substrates
    • Colorimetric and chemiluminescent enzyme substrates for detection

 Complete the form below to receive your ELISA Development Handbook