Mass spectrometry, trypsin digestion & electrophoresis sample preparation

Start with clean samples for improved mass spec data

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The guide focuses on the preparation of proteins for identification by mass spectrometry, from initial lysis to peptide generation. For peptide generation the guide reviews are highly active mass spectrometry grade trypsin and self contained kits for in gel peptide generation.

The Mass Spectrometry Sample Preparation Handbook features the following sections:

• Cell Lysis & Protein Extraction
  • A large selection of reagents specifically designed to be compatible with 2D electrophoresis and mass spectrometry, including highly purified proteomic detergents to limit protein modifications
• High Abundant Protein Removal
  • Aid in identification of low abundant proteins by removing the highly abundant albumin and immunoglobulins
• Protein Fractionation & Enrichment
  
  • Enrich specific protein pools (phosphoproteins, glycoproteins and more) or fractionate by various properties to simplify the protein pool to be analyzed
• Clean-up & Concentration
  • A crucial step to ensure that all 2D electrophoretic and mass spec interfering agents are removed, including detergents.
• Electrophoresis Separation
  • From protein markers, 2D accessories to mass spec compatible stains
• Peptide Generation
  
  • A selection of proteases, including our mass spectrometry compatible trypsin, sequencing grade trypsin and in gel digestion kits