Protein Purification Handbook for Recombinant and Native Proteins

Affinity chromatography resin production, recombinant protein purification

Protein_Purification_HandbookDOWNLOAD NOW

A handbook for purification of both native and recombinant proteins using affinity chromatography, protein tagging/ labeling and immunoprecipitations.  The handbook also features tools for the removal of contaminating reagents, including detergents.

The Protein Purification Handbook features the following sections:

  • General Protein Purification
    • Featuring a proprietary electroelution device to isolate any protein from electrophoresis gels
  • Affinity Chromatography Resin Production
    • Activated resins designed to bind to various groups to allow simple generation of affinity chromatography columns.  Resins are activated to bind to:
      • Sulfhydryls
      • Amines
      • Carboxyls
      • Carbohydrates
      • Active hydrogens
  • Recombinant Protein Purification
    • Resins and kits for the isolation of recombinant proteins with a specific protein tag.  Tags featured include:
      • CBP (Calmodulin Bnding Protein)
      • GST (Glutathione S-Transferase)
      • 6X His (Large selection of IMAC tools)
  • Biotin Purification
    • Resins and tools to purify biotinylated proteins and other biomolecules
  • Antibody Purification
    • Protein A, Protein G and Protein A-G to isolate immunoglobulins
    • Other antibody purification tools, including our Pearl resin and thiophilic resins
  • Additional Affinity Purification
    • Tools for the isolation of specific protein groups, including:
      • Glycoproteins
      • Lectins
      • Phosphoproteins
      • C-Reactive Proteins
  • Immunoprecipitation
    • Four kits for immunoprecipitation, including co-immunoprecipitation
  • Contamination Removal
    • A key step to allow sample to be compatible with downstream applications.  Features protease removal resins, spin format desalting and buffer exchange columns and detergent and endotoxin removal tools
  • Protein Purification Accessories
    • Disposable columns for spin or gravity flow purification

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