Protein Purification Handbook for Recombinant and Native Proteins
Affinity chromatography resin production, recombinant protein purification
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A handbook for purification of both native and recombinant proteins using affinity chromatography, protein tagging/ labeling and immunoprecipitations. The handbook also features tools for the removal of contaminating reagents, including detergents.
The Protein Purification Handbook features the following sections:
- General Protein Purification
- Featuring a proprietary electroelution device to isolate any protein from electrophoresis gels
- Affinity Chromatography Resin Production
- Activated resins designed to bind to various groups to allow simple generation of affinity chromatography columns. Resins are activated to bind to:
- Sulfhydryls
- Amines
- Carboxyls
- Carbohydrates
- Active hydrogens
- Activated resins designed to bind to various groups to allow simple generation of affinity chromatography columns. Resins are activated to bind to:
- Recombinant Protein Purification
- Resins and kits for the isolation of recombinant proteins with a specific protein tag. Tags featured include:
- CBP (Calmodulin Bnding Protein)
- GST (Glutathione S-Transferase)
- 6X His (Large selection of IMAC tools)
- Resins and kits for the isolation of recombinant proteins with a specific protein tag. Tags featured include:
- Biotin Purification
- Resins and tools to purify biotinylated proteins and other biomolecules
- Antibody Purification
- Protein A, Protein G and Protein A-G to isolate immunoglobulins
- Other antibody purification tools, including our Pearl resin and thiophilic resins
- Additional Affinity Purification
- Tools for the isolation of specific protein groups, including:
- Glycoproteins
- Lectins
- Phosphoproteins
- C-Reactive Proteins
- Tools for the isolation of specific protein groups, including:
- Immunoprecipitation
- Four kits for immunoprecipitation, including co-immunoprecipitation
- Contamination Removal
- A key step to allow sample to be compatible with downstream applications. Features protease removal resins, spin format desalting and buffer exchange columns and detergent and endotoxin removal tools
- Protein Purification Accessories
- Disposable columns for spin or gravity flow purification
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