Western Blotting Handbook and Troubleshooting Guide

Protein Electrophoresis and Western Blot Transfer Solutions

Western Blotting and Protein ElectrophoresisDOWNLOAD NOW

The Western blot handbook features a complete overview of products for protein electrophoresis, Western transfer and then subsequent blocking and immunoblotting.  In addition, the handbook has an in-depth troubleshooting section to help solve issues with Western blotting. 

The Western Blot Handbook features the following sections:

  • Protein Electrophoresis
    • Reagents to prepare your own gels, protein ladders and markers, electrophoresis running buffers and sample electrophoresis clean up reagents
  • Protein Transfer
    • Proprietary transfer pads to improve and reduce Western transfer quality and time respectively.  
    • High efficient transfer buffers
    • Reversible membrane stains to ensure optimal transfer
  • Blocking Agents
    • Non-animal blocking agents
    • Non-sera animal protein blocking agents
    • Protein blocking agents
  • Wash Buffers
    • PBST and TBST wash buffers
  • Secondary Antibodies
  • Chemiluminescence Detection
    • ECL detection kits for picogram to femtogram detection levels for horseradish peroxidase (HRP) and alkaline phosphatase (AP)
  • Chromogenic Detection
    • For alkaline phosphatase and horseradish peroxidase
  • Stripping Solutions for Reprobing
  • Troubleshooting Section
    • Solutions to the following common Western blotting problems
      • Uniform high background on membranes
      • Blotchy or Speckled High Background
      • Weak or no signal
      • Non-Specific bands
      • Diffuse bands
      • Ghost or hollow bands or brown/yellow bands on membrane
      • Blank areas on blot

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