EDC Conjugation Protocols

EDC.jpgBiochemical Conjugation, Modification and Labeling of Oligonucleotide 5’ Phosphate Groups with EDC

Tips, tricks and protocols for successful coupling with EDC

The heterobifunctional, zero-length carbodiimide crosslinker EDC is a versatile tool that can be used with Imidazole to modify, label, immobilize or bioconjugate oligonucleotides, DNA and RNA at their 5’ phosphate groups for a variety of applications:

  • Conjugate carboxyl to amine groups in peptides and proteins
  • Oligonucleotide/DNA/RNA labeling through 5’-phosphate groups
  • Couple haptens or peptides to carrier proteins
  • Immobilize peptides for affinity purification
  • Crosslink proteins to carboxyl surfaces and beads
  • Convert carboxyls to amine-reactive Sulfo-NHS esters
  • Activate nanoparticles to amine-reactive Sulfo-NHS esters
Download application note for specific protocols