G-Biosciences responds to the Coronavirus (COVID-19) pandemic
Research reagents to inform the fight against a global crisis
December 2019 a novel Coronavius (COVID-19) began to spread from Wuhan, Hubei province, China. This novel virus within weeks would begin it's global journey, infecting more than 300,000 and killing more than 15000 by the end of March 2020 (https://www.who.int/emergencies/diseases/novel-coronavirus-2019/situation-reports/). Resembling a similar outbreak in 2002, COVID-19 is severe acute respiratory syndrome (SARS) inducing Coronavirus that likely was introduced to humans through the consumption of bat meat (Zhou et al. 2020).
Coronaviruses are single stranded RNA viruses that generally transmit through saliva droplets or discharge from the nose of an infected person. As previously mentioned, many Coronavirus are capable of zoonoses and are contracted by humans from their natural reservoir such as bats, COVID-19 is a novel Coronavirus and has not been fully characterized. ACE2 however has been identified as the receptor required for endocytosis of the virus.
In response to the COVID-19 pandemic, G-Biosciences is highlighting products useful for the study of RNA viruses and their pathogenesis. For more information about additional custom capabilities available to the research community please email firstname.lastname@example.org.
RNA-Conserve™RNA-Conserve™ Solution is designed to store and preserve RNA in various samples such as cells, tissue, plant, soil, blood etc before RNA extraction. RNA-Conserve™ Solution is non-toxic aqueous solution that permeates tissues and cells and inactivates RNases to preserve the RNA. RNA-Conserve™ Solution eliminates the need to freeze the samples or immediately process the sample to extract RNA. RNA extracted from samples stored in RNA-Conserve™ Solution is intact in its quality and quantity. FeaturesRNA stabilization solution that permeates cells and tissues and inactivate the RNAses.Convenient: No need to immediately freeze or process sample for RNA extraction. Simply add RNA-Conserve™ Solution and store the sample.Stable: Tissues and cells stored in the solution are stable for 1 day at 37°C, for 1 week at room temperature (~25°C), one month at 4°C and indefinitely at -20°C.Mobility: Easy to carry and store field samplesCompatible: with various RNA extraction methods and kits.Application(s)RNA-Conserve™ Solution is designed to store and preserve RNA in various samples such as cells, tissue, plant, soil, blood etc before RNA extraction without the need to freeze samples. RNA extracted from samples stored in RNA-Conserve™ Solution is compatible with downstream applications such as RT-PCR, norther blot, Real time RT-PCR, sequencing and northern blot
GET™ total RNA purification kitThe GET™ Total RNA kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA Spin Columns. The <60-minute protocol is simple; after homogenization, RNA is bound to the GET™ RNA Spin Columns and washed. The protocol provides an option to remove contaminating DNA with a single DNase treatment. Finally RNA is eluted from the column. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays. Three step protocol:Arrest™ Extraction Buffer lyses samples instantly destroying RNase activity.GET™ RNA Spin Columns captures RNA.After a brief washing step, pure and protein free RNA is eluted. FeaturesRapidly inhibit destructive RNasesStrong chaotropic buffer for rapid RNA releaseHigh affinity RNA binding spin columnsOptional DNase treatmentCompatible with numerous species and tissuesSuitable for animal and plant tissues, cultured cells, blood and bacteriaSuitable for 50 x 50mg extractions ApplicationsFor the extraction and purification of protein and DNA free RNA.
Immunotag™ ACE2 Polyclonal AntibodySize 50 µg, 100 µg Conjugation:HRP, Biotin, FITC, Alexa Fluor® 350, Alexa Fluor® 405, Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 594, Alexa Fluor® 647 Target Protein:ACE2Clonality: Polyclonal Storage/Stability: -20°C/1 year Application: WB,ELISA Recommended Dilution Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. Concentration: 1 mg/ml Reactive Species: Human,MouseHost Species: Rabbit Immunogen: The antiserum was produced against synthesized peptide derived from human ACE2. AA range:416-465 Specificity: ACE2 Polyclonal Antibody detects endogenous levels of ACE2 protein.Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
RNaseOUT™RNaseOUT™ is uniquely formulated to destroy and remove RNases on contact. Simply spray and rinse. RNaseOUT™ is non-toxic and residue free. RNaseOUT™ allows common equipment to be safely and confidently used for all RNA work. RNaseOUT™ is supplied in 250ml easy to apply spray bottles. Simply spray on items or area to decontaminate and rinse. Spraying prevents waste and is designed to uniformly cover the application area. For economy, refilling spray bottles, or cleaning large items, RNaseOUT™ is also available in 1 liter refill bottles.
Tri-Xtract™Tri-Xtract™ is a convenient, ready-to-use reagent designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridization, in-vitro translation, RNase protection assays and poly (A+) selection. Tri-Xtract™ is a monophasic solution of phenol and guanidine thiocyanate that maintains the integrity of the RNA during cell disruption and homogenization, the addition of chloroform results in the separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase. The total RNA is recovered by isopropyl alcohol precipitation. The DNA and proteins can also be recovered by sequential precipitation from the organic phase. Tri-Xtract™ is suitable for the isolation of total RNA, including mRNA, hnRNA, ribosomal RNA and tRNA, from small quantities of tissues (50-100mg) and cells (5 x 106) from human, animal, plant, yeast, bacterial and viral origin. Total RNA is isolated in under an hour and the subsequent isolation of DNA and protein in less than three hours.
Silica Magnetic BeadsG-Biosciences Silica Magnetic Beads are Fe3O4 magnetic beads coated with a silicon dioxide (SiO2) layer. Since silica is able to bind to the nucleic acids, G-Biosciences Silica Magnetic Beads serve as a simple and efficient tool for plasmid DNA purification for transfection or sequencing applications, genomic DNA purification for research or clinical applications, RNA purification for qPCR analysis, or PCR product clean-up for downstream analysis.